NP870: An epochal incubator specialized for cell production
CO2 incubators were originally used for research, but as the market demands of regenerative medicine and cell therapy rises, the cases of being operated for manufacturing purposes are increasing. However, the current products are not necessarily optimized for operation in specific cell processing facilities (CPC / CPF) based on GMP (Good Manufacturing Practice). We have developed a CO2 incubator as “a device for cell production” that satisfies the requirements of manufacturability, reliability and reduced management costs, which are based on many years of experience and knowledge.
Improve productivity and Expand a range of applications
Cell production has a wide variety of culture method and scale. Until now, middle-sized products with a chamber volume of 150 to 180 L have often been used for cell production in specific cell processing facilities. NeXCell P870 (NP870) is the largest class of model with a chamber volume of 870L. It has approximately 5 times the capacity of middle-sized products, and can significantly improve productivity (= size and quantity of culture vessels).
Excellent temperature uniformity and recovery performance
Conventionally, since large CO2 incubators have the huge size of chamber, they have had various issues such as temperature uniformity, recovery performance when opening and closing doors, and excessive condensation inside the chamber due to humidification. “Despite being large, achieve performance and quality comparable to middle size” We worked on the theme of not compromising on performance in the development stage of NP870. After various trials and errors, we achieved best-in-class performance with our own air circulation system and heater control technology.
Suitable for CellSTACK / Cell Factory demands
As an efficient way for scale up of adherent cell culture, demand for multi-layer culture flask (stack plates) such as CellSTACK (Corning) and Cell Factory (ThermoFisher Scientific) is increasing. In the case of a 10-layer vessel (CF10 / CS10), the weight of the vessel including the culture medium is about 5 kg. The reinforced shelf for NP870 can hold six 10-layer vessels. The shelf has a rugged design that guarantees robustness and flatness without sagging even by loads up to 30kg or more. In addition, the sophisticated temperature control and air circulation system achieves the excellent temperature uniformity and recovery performance, which is comparable to the no-load condition even when the culture vessel is fully loaded.
The biggest concern of the CO2 incubator is the propagation of mold and various germs, that is to say “contamination”. No matter how much attention you pay, it is an unavoidable problem to bring them in from the installation environment. NP870 comes standard with a special HEPA filter that cleans the circulating atmosphere inside the chamber. This filter using PTFE, which is different from the conventional one, has the property of being able to withstand high humidity with low pressure loss (hydrophobicity). The atmosphere in the chamber reaches ISO class 5 (class 100) cleanliness, equivalent to a safety cabinet or clean bench, in just 10-15 minutes after opening and closing the door. In addition, by using WATERBAG developed by ourselves, the replacement work of humidification water is made easy, and clean operation without dispersing mold and various bacteria of humidification water has become possible.
HEPA filter(left）and WATERBAG(right)