Freezing and Thawing experiment for large volume of Human iPS cell-derived cardiomyocytes

Upon the development of cell-processed products and regenerative medical products, scale-up of cell culture process is considered to be essential. However, there is concern that to achieve scale-up could affect the efficiency of cell culture or other processes. Besides, in case of using aoutologous cells, the cells can’t be used for threatment immediately because it takes long time to cultivate after collection. For this reason, allogenic cells (ES cells, iPS cells), cells donated by donors have been attracting attention recently. By collecting and cultivating a lot of cells from donors beforehand, they can be used for treatment sooner. This time we evaluate cryopreservation of human iPS cell-derived cardiomyocytes for treatment volume based on the success of cryopreservation of undifferentiated iPS cells at preliminary experiment. Also, we used CryoMACS Freezing Bag and ThawSTAR CB the same as preliminary experiment.