{"id":4101,"date":"2026-01-28T12:49:25","date_gmt":"2026-01-28T03:49:25","guid":{"rendered":"https:\/\/www.wakenbtech.co.jp\/eng\/?post_type=_blog&p=4101"},"modified":"2026-03-25T16:24:02","modified_gmt":"2026-03-25T07:24:02","slug":"optimizing-large-scale-cell-culture-forcibly-gas-infusion-functionality-of-the-maxcell-mlf-incubator","status":"publish","type":"_blog","link":"https:\/\/www.wakenbtech.co.jp\/eng\/_blog\/optimizing-large-scale-cell-culture-forcibly-gas-infusion-functionality-of-the-maxcell-mlf-incubator\/","title":{"rendered":"Optimizing Large-Scale Cell Culture: Forcibly gas infusion functionality of the MaxCell MLF Incubator"},"content":{"rendered":"\n\n\n\n\n\n<\/head>\n\n

We have received many inquiries regarding our MaxCell MLF, a large volume incubator designed for large-scale cell culture, so far. While we have previously introduced the MLF incubator on this blog, we would like to dive deeper into one of its most critical features: Forcibly gas infusion functionality.
\nThe MLF series is specifically optimized for large-scale culture vessels such as CellSTACK and CellFactory (CF40\/52). MLF1 can accommodate 4 pcs of 40-layer vessels, while MLF2 can accommodate 8.
\nWhen using standard 36-layer HYPERStack, MLF1 can accommodate 8 pcs, and MLF2 can accommodate 16.
\nThe dedicated flask holders (Item No. MLF-UW) can be customized (for an additional fee) to support specific vessels, such as 36-layer HYPERStacks.<\/p>\n

The standout feature of the MLF series is the forcibly gas infusion functionality (FA model). Unlike other standard large-capacity incubators that regulate CO2 concentration only within the chamber, the MLF FA model uses a pump to actively inject pre-mixed CO2 gas directly into the flasks.<\/p>\n\n

Why is this functionality necessary and important?<\/p>\n\n

In a standard incubator, even if the chamber is set to 5% CO2, the gas only reaches the top 2\u20133 layers of a 40-layer. Without forced infusing, the lower layers rarely reach the target (setting) CO2 concentration. This results in inconsistent culture environments between the top and bottom layers.
\nIn large-scale production using 40-layer flasks, CO2 consistency is frequently overlooked. However, inconsistent environments lead to variations in cell quality, which could make difference in the integrity of your results or production batch.
\nBy using the forcibly gas infusion function, CO2 gas (e.g. 5%) is delivered directly into the vessels (see image below). This ensures that every layer reaches close to 5%, effectively eliminating environmental disparities and ensuring uniform cell quality across all layers.<\/p>\n\n